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KMID : 0615719990160030255
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Jouranl of Korean Acupuncture and Moxibustion society
1999 Volume.16 No. 3 p.255 ~ p.268
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The effect of OK aqua-acupuncture on WBC , fibroblast , collagen responses to Rheumatoid arthritis in mice
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Abstract
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OBJECTIVE : Rheumatoid arthritis(RA) is a chronic inflammatory autoimmune disease characterized by an intense immunological activity in the synovial tissue. WBC has taken charge of an important defense mechanism in the rheumatoid chronic inflammatory reaction. Neutrophil is increased by acute inflammation. Increase of lymphocyte show existence of immune response. Monocyte in synovial membrane provoke inflammatory reaction. Fibrosis and fibroblast in articular synovial membrane distroy articular cartilage, is appeared beginning of arthritis. The increase of Collagen in articular synovial membrane implies rheumatoid inflammatory reaction. RA is referred to the category of severe and migratory arthralgia in oriental medicine. Lipopolysaccharide(LPS)-induced arthritis, an animal model of autoimmune inflammatory polyarthritis, has features similar to RA. The aim of this study is to examine the effects of OK aqua-acupuncture on hematological change and in articular synovial membrane in mice with LPS induced arthritis.
METHODS : All the BALB/c mice used in this study were bred and maintaned in our pathogen-free mouse facility and were 6weeks of age at the start of the experiment. The experimental model of arthritis was induced by injecting 300㎍/㎏LPS to mice knee joint. The OK A-A was injected into Ya′ngli^gqua′n(G_34) of mice every other day for 14 days. Neutrophil, lymphocyte, monocyte counts in peripheral blood were measured to estimates inflammatory response. Fibrosis and fibroblast appear in synovial membrane after stimulation with OK A-A. Results obtained from OK A-A stimulation group were compared to the results from control group, which was not treated.
RESULTS :
1. The neutrophil counts in peripheral blood were increased (p < 0.05) compared to control group in seven, fourteen days after arthritis onset.
2. The lymphocyte counts in peripheral blood were decreased (p < 0.05) compared to control group in fourteen days after arthritis onset.
3. The monocyte counts in peripheral blood were decreased (p < 0.05) compared to control group in three, seven, fourteen days after arthritis onset.
4. The fibrosis and fibroblast in articular synovial membrane were decreased compared to control group in fourteen days after arthritis onset.
5. The collagen in articular synovial membrane were decreased compared to control group in fourteen days after arthritis onset.
CONCLUSIONS : OK A-A stimulation decreased inflammatory responses to LPS induced arthritis in mice. OK A-A stimulation may have treatment effects in rheumatoid arthritis disease.
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